Leica tcs sp8 user manual

Leica tcs sp8 confocal microscope flow cytometrycell. Please log on with your account only and do not share your. Page 1 training guide for leica sp8 confocalmultiphoton microscope las af v3. In addition, the leica tcs sp8 features 3 hyds hybrid detectors combining the characteristics of a classic pmt with a highly sensitive avalanche photodiode and 1 pmt which can measure real time response of timecritical sequences due to its fast scan speed. The leica tcs sp8 dls is a digital light sheet microscope, which is fully integrated into the leica tcs sp8 confocal microscope for the observation of. Aobs and wll 470nm670nm white light, pulsed laser source leica tcs sp8 x perfectly matches the wavelength of any fluorophore in the visible spectrum. This application letter describes work with the fret ab wizard 2. Double click on the las x software found on the desktop. By tuning both excitation and detection, complete excitation emission spectra can be acquired. Training guide for leica sp8 confocalmultiphoton microscope. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. All rights to this document are held by leica microsystems cms gmbh. Microscopes microscope stand options upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options speci.

Check the scheduling calendar to see if anyone else has signed up to use the leica sp8 after. Since the lcs software is based on the user administration of windows. Leica dmi8 microscope user manual manual pdf viewdownload. The gain setting as discussed elsewhere in this manual controls. Fret ab and fret ratio leica sp8 university of houston. The first step is to set the lasersdetectors for the fret donor.

Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user. Leica sp8 tcs users manual v2 university of oklahoma. While the leica tcs sp2 was stateoftheart when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity. Leica tcs sp8 wll confocal smd microscope wellcome. Unlike our previous instrument, it has a number of hardware features, specifically the.

Tcs sp8 mp with dmi6000 and ir laser without dispersion compensation. Las af live data mode recording of manual and automated work. Leica sp8 mp scientific center for optical and electron. Description leica tcs sp8 has been designed for confocal microscopy with optimal photon efficiency and high speed. Usermanual for leica sp8 confocal universitetet i bergen. Leica histocore arcadia h laboratory equipment user manual. Leica tcs sp8 technical documentation life science research division. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. View online user manual manual for leica dmi8 microscope or simply click download button to examine the leica dmi8 guidelines offline on your desktop or laptop computer. Leica tcs sp8 startup shutdown university of houston. A pulsed 440nm laser and a continuous wave 405nm laser line is included for imaging of uvexcitable dyes. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018 pdf, 717 kb press releases.

Leica dm2500 upright fluorescent microscope manuals. If you do not require high speed scanning leave resonant set to off. This portion will quickly familiarize users with the layout and control of the leica lasx software interface. If you have any suggestions or improvements for this user manual, please contact the leica branch office in your country. Theres also the full leica sp8 user manual plus the other leica sp8 confocal manuals. Up to eight excitation lines can be used simultaneously. It achieves this level of performance using highspeed resonant scanning with imaging formats of up to 2496 x 2496 pixels. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave computer and hardware running.

It is recommended setting up a separate user id for each user system administrator. Dichroic color tab 1 allows the user to switch between brightfield bf or fluorescence fluo 2. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. Sted super resolution leica dm5000 cs leica dm6000 cs leica dm6000 cfs inverted leica dmi6000 cs leica dmi6000 cel. Tcs sp8 smd system variants with white light laser. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. The existing multiuser confocal system, a leica tcs sp2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. The leica tcs is a universal laser scanning confocal microscope system for the. Leica sp8 confocal with white light laser, falcon flim.

If you are the last user fo the day, turn off the complete system incl. This allows acquisition of excitation and emission spectra. Starting the lcs software on the leica tcs sp5 confocal microscope. Manual x, y and z stage control is via the knobs attached to the. If you turn it on you will not be able to use slower speeds. View and download leica tcs sp8 mp user manual online. How to do a tile scan with the leica dvm6 digital microscope duration. The leica tcs sp8 sted 3x leica microsystems, germany is a highly versatile and advanced high end system from leica microsystems equipped with three hyds, two pmts and one transmission detector, tandem max speed 1800 hz and resonance 8000 hz scanners, lasers throughout the visible range, 2 depletion lasers for sted, and a sted 3d module. In this example i have used a linked cfpyfp fret sensor with fret as its default state, expressed in the mushroom body of an adult drosophila brain. Then turn on the power supply for the scanner 1 and the lasers 2 by. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Leica tcs sp8 with compact supply unit and upright stand 1 research microscope 2. Leica tcs sp8 confocal microscope system andrew lackner. Fill out the logbook and comment on the performance of the system or failure description.

Nih image free image processing and analysis program for. Leica tcs sp8 lives up to the high demands of this microscope discipline and can adapt as research evolves. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. The most important tools are located directly above the time line figure 9.

Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. Leica dmi6000 inverted microscope brochure leica dmi6000 inverted microscope instruction manual. Leica sp8 confocal with white light laser, falcon flim, sted and lightning deconvolution. Leica tcs sp8 sted 3x confocal molecular imaging center. Select the fretab wizard from the tcs sp8 pulldown menu. Manual stage or custom made setup for animal fixation. Las x live data mode recording of manual and automated workflows, trigger functions, complex timelapse series. Further help is available via inprogram help within the leica lasaf software. Leicatcs the confocal microscope system for highest demands in multi user facilities.

User guide to the ibif leica tcs sp8 mp confocal microscope. The sp8 lightning allows you to perform proper sampling over a specimen area at least six times larger than with any other confocal system. A guide through the live data mode live data mode with leica tcs sp8 7 4 tools in detail 4. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. Leica tcs sp8 x with white light laser wll perfectly matches the wavelength of any fluorophore. Leica sp8 user manuals and brochures download the leica tcs sp8 quick start poster and cores abridged instructions for getting started here. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. The owneroperator and user of this product are responsible for proper. Leica sp8 confocal, sted, flim system microscopy and.

Confocal application letter 3 fret with tcs sp8 fret wizards in leica application suite advanced fluorescence las af in the las af menu bar 1 you can find two wizards for performing fret experiments. Follow the procedure for start up and log on as posted in the lab. User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. For cfp, 458nm is the best of our available laser wavelengths. Leica lasx is the standard software used for the leica microscopes. Training guide for leica sp8 baylor college of medicine. User guide to the ibif leica tcs sp8 mp confocal microscope this version. This user manual specifies names of products or services that are trademarks or registered. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. This will create individual directories that can be viewed only by the respective user. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. Introduction the ibif leica confocal microscope is made available on a feeforusehour basis to all users who have been trained. The user can select the appropriate option for his or her application 1.

Quick start leica tcs sp8 florida state university. Turn on the fluorescent lamp top left of the computer monitor. Leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars imaging native specimens without dyes 32 leica hcs a amplify the power of screening 34 leica tcs sp8 smd learn more from single molecules 36 find the right leica tcs sp8 for your research 38 captures and acknowledgements 39. With this spectral information, any dye can be optimally excited with minimum crossexcitation and specimen.